Antigen

Antigen Class

TFs and others

Antigen

GFP

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

embryonic stem cells

cell type

embryonic stem cells

culture conditions

2i+LIF

strain

129/Ola

chip antibody

Anti-GFP

genotype/variation

Mta2-GFP knocked in to the endogenous Mta2 locus

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations were carried out as previously outlined (Reynolds et al., 2012). ChIP-seq libraries were prepared using the NEXTflex Rapid DNA-seq kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

66733684

Reads aligned (%)

97.3

Duplicates removed (%)

18.8

Number of peaks

18055 (qval < 1E-05)

mm9

Number of total reads

66733684

Reads aligned (%)

97.0

Duplicates removed (%)

18.7

Number of peaks

17994 (qval < 1E-05)