Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
embryonic stem cells
cell type
embryonic stem cells
culture conditions
2i+LIF
strain
129/Ola
chip antibody
input
genotype/variation
Mta1-FLAG knocked into endogenous Mta1 locus

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin immunoprecipitations were carried out as previously outlined (Reynolds et al., 2012). ChIP-seq libraries were prepared using the NEXTflex Rapid DNA-seq kit (Illumina)

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
68915292
Reads aligned (%)
97.3
Duplicates removed (%)
17.1
Number of peaks
580 (qval < 1E-05)

mm9

Number of total reads
68915292
Reads aligned (%)
97.1
Duplicates removed (%)
17.0
Number of peaks
691 (qval < 1E-05)

Base call quality data from DBCLS SRA