Antigen

Antigen Class

TFs and others

Antigen

Epitope tags

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

embryonic stem cells

cell type

embryonic stem cells

culture conditions

2i+LIF

strain

129/Ola

chip antibody

anti-FLAG

genotype/variation

Mta1-FLAG knocked into endogenous Mta1 locus

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin immunoprecipitations were carried out as previously outlined (Reynolds et al., 2012). ChIP-seq libraries were prepared using the NEXTflex Rapid DNA-seq kit (Illumina)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

53310579

Reads aligned (%)

96.9

Duplicates removed (%)

23.0

Number of peaks

9151 (qval < 1E-05)

mm9

Number of total reads

53310579

Reads aligned (%)

96.6

Duplicates removed (%)

22.9

Number of peaks

8944 (qval < 1E-05)