Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Natural Killer T-Cells

MeSH Description

A specific natural killer T-cell subtype that expresses an invariant T-cell receptor alpha-chain.

Sample

source_name

CD56Bright NK cells

cell type

CD56Bright NK

individual

Donor 7

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Lysates were clarified from MNase treated nuclei and histone-DNA complexes were isolated with antibody. ATAC-seq were processed as per manufacturers instructions (Illumina FC-121-1030). RNA was purified using Rneasy Plus Microkit, and prepared using SMARTer Universal Low Input RNA kit (Clontech) Nextera DNA Library Preparation Kit, Illuminia

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

52366502

Reads aligned (%)

90.3

Duplicates removed (%)

64.0

Number of peaks

43129 (qval < 1E-05)

hg19

Number of total reads

52366502

Reads aligned (%)

90.1

Duplicates removed (%)

64.2

Number of peaks

42971 (qval < 1E-05)