Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Neural

Cell type

Neurofibroma

MeSH Description

A moderately firm, benign, encapsulated tumor resulting from proliferation of SCHWANN CELLS and FIBROBLASTS that includes portions of nerve fibers. The tumors usually develop along peripheral or cranial nerves and are a central feature of NEUROFIBROMATOSIS 1, where they may occur intracranially or involve spinal roots. Pathologic features include fusiform enlargement of the involved nerve. Microscopic examination reveals a disorganized and loose cellular pattern with elongated nuclei intermixed with fibrous strands. (From Adams et al., Principles of Neurology, 6th ed, p1016)

Sample

source_name

Primary Nf1fl/fl;DhhCre mouse neurofibroma cells

stain

C57BL/6-I129

genotype/variation

Nf1fl/fl;DhhCre

cell type

primary neurofibroma cells

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

20476882

Reads aligned (%)

95.3

Duplicates removed (%)

22.3

Number of peaks

365 (qval < 1E-05)

mm9

Number of total reads

20476882

Reads aligned (%)

95.0

Duplicates removed (%)

22.4

Number of peaks

302 (qval < 1E-05)