Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Cardiovascular
Cell type
Coronary artery smooth muscle
NA
NA

Attributes by original data submitter

Sample

source_name
coronary artery smooth muscle cells
cell type
coronary artery smooth muscle cells
lines
1508
passage
4-7
antibody
anti-H3K27ac(ab4729)
genotype/variation
TCF21 knockdown

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated cells and protein-DNA complexes were isolated with antibody. Libraries were prepared with KAPA Hyper Prep kit (KK8502) per standrad instruction.

Sequencing Platform

instrument_model
HiSeq X Ten

hg38

Number of total reads
167781682
Reads aligned (%)
55.9
Duplicates removed (%)
62.3
Number of peaks
16691 (qval < 1E-05)

hg19

Number of total reads
167781682
Reads aligned (%)
55.4
Duplicates removed (%)
62.9
Number of peaks
16110 (qval < 1E-05)

Base call quality data from DBCLS SRA