Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

NT2-D1

Tissue

testis

Lineage

inner cell mass

Description

malignant pluripotent embryonal carcinoma (NTera-2), "The NTERA-2 cl.D1 cell line is a pluripotent human testicular embryonal carcinoma cell line derived by cloning the NTERA-2 cell line." - ATCC. (PMID: 6694356)

Sample

source_name

human embryonic carcinoma cells

cell line

NT2

cell type

human embryonic carcinoma cells

chip antibody vendor

Millipore

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

27512726

Reads aligned (%)

99.0

Duplicates removed (%)

51.9

Number of peaks

664 (qval < 1E-05)

hg19

Number of total reads

27512726

Reads aligned (%)

98.2

Duplicates removed (%)

53.5

Number of peaks

774 (qval < 1E-05)