Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Liver

Cell type

Hepatocytes

MeSH Description

The main structural component of the LIVER. They are specialized EPITHELIAL CELLS that are organized into interconnected plates called lobules.

Sample

source_name

H3K27ac ChIP-seq MPH DMSO

strain background

C57Bl6

genotype/variation

WT

age

adult

Sex

male

cell type

mouse primary hepatocytes (MPH)

treatment

vehicle (0.04% DMSO) for 4hrs

chip antibody

H3K27ac (Active Motif, Cat. # 39685)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was performed as described in Flajollet S., Lefebvre B., Rachez C., and Lefebvre P. 2006. Distinct Roles of the Steroid Receptor Coactivator 1 and of MED1 in Retinoid-induced Transcription and Cellular Differentiation. J. Biol. Chem. 281:20338-20348. Diagenode MicroPlex Library Preparation kit v2

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

48667226

Reads aligned (%)

85.5

Duplicates removed (%)

21.6

Number of peaks

19939 (qval < 1E-05)

mm9

Number of total reads

48667226

Reads aligned (%)

85.3

Duplicates removed (%)

21.6

Number of peaks

19966 (qval < 1E-05)