ChIP-Atlas: SRX5007215

Antigen

Cell type Class: Blood
Cell type: Macrophages
MeSH Description: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: The CD45+CD11c+SiglecF+ lung alverolar macrophages (AMs) were sorted by FACS Aria II flow cytometer. Cell pellets from the freshly isolated AMs were directly frozen at -80°C. The samples were then submitted to EpiGentek for ChIP, library preparation, and sequencing. The cells were fixed with 1% formaldehyde and chromatin was isolated using ChromaFlashTM Chromatin Extraction Kit. Chromatin was sheared using the Episonic2000 Sonicatin System. ChIP was performed using an antibody against HDAC3, H3K27ac or H3K9ac on the chromatin and Input DNA (without immunoprecipitation) was used as background. After adapter linking, library was size-selected (100-300 bp) and PCR-amplified. Ten nanomolar of sample libraries were provided for next generation sequencing.