Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Spleen

Cell type

Spleen

MeSH Description

An encapsulated lymphatic organ through which venous blood filters.

Sample

source_name

spleen

strain

C57BL/6

antibody

H3K27me3

extra protocol

ChIPmentation (see De Bock et al 2018; Vanden Bemtp et al 2018)

genotype/variation

JAK3 mutant + Suz12 inactivated - CD8+ SP

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

all libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

40685180

Reads aligned (%)

97.0

Duplicates removed (%)

17.0

Number of peaks

706 (qval < 1E-05)

mm9

Number of total reads

40685180

Reads aligned (%)

96.8

Duplicates removed (%)

17.0

Number of peaks

697 (qval < 1E-05)