GSM3466348: DNase-seq, BAT adipocytes; Mus musculus; DNase-Hypersensitivity
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
DNase-seq
Antigen
DNase-Seq
Cell type
Cell type Class
Adipocyte
Cell type
Brown adipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
BAT-derived primary adipocytes
strain
NMRI
Sex
male
age
4-5 weeks
tissue
brown adipose tissue
cell type
Primary in vitro differentiated SVF cells
Sequenced DNA Library
library_strategy
DNase-Hypersensitivity
library_source
GENOMIC
library_selection
DNase
library_construction_protocol
DNase I digestion was performed in eWAT, iWAT and BAT-derived adipocytes on ~10,000,000 million nuclei, essentially as previously described (Siersbæk et al., 2011, EMBO J, 30(8):1459-72), except that the mature adipocytes were harvested directly in Buffer A (15 mM Tris–HCl pH=8, 15 mM NaCl, 60 mM KCl, 1 mM EDTA, 0.5 mM EGTA, 0.5 mM spermidine) and 120 U/ml DNase I (Roche, cat# 11 284 932 001) was used for digestion. DNase-seq libraries were constructed according to the manufacturer's instructions (Illumina) as described in (Nielsen R, Mandrup S, 2014 Genome-Wide Profiling of Transcription Factor Binding and Epigenetic Marks in Adipocytes by ChIP-seq. Methods in Enzymology 2014, Vol. 537, pp. 261-279).