GSM3464072: ECr/VCr atl2 2.3; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD4+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
peripheral blood
cell type
activated CD4+ T cells
activation time
3 days
activation protocol
anti-CD3/CD28 in presence of IL2
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
50,000 cells were lysed and transpositions were performed using transposase mixture (Nextera DNA library prep kit, Illumina), supplemented with 0.01% digitonin (Promega). Transposition reactions were incubated for 30 min at 37°C in a ThermoMixer with agitation at 300rpm. DNA was purified using the MinElute Reaction Cleanup kit (Qiagen) libraries were amplified using NEBnext PCR master mix with Illumina primers for ATACseq according to Illumina's instructions