Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Blood

Cell type

Bone Marrow Cells

MeSH Description

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Sample

source_name

Bone marrow

strain

C57BL/6

tissue

Bone marrow

cell type

HoxB8

vector

CSF3R.T618I and CEBPA.v314vw

chip marker

H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed and lysed to extract chromatin. Fixed chromatin was sheared and appropriate antibodies were used to perform the pull down. Aliquotes of the sheared chromatin were used as input. ChIP and INPUT libraries were prepared using the NEB Ultra II kit (E7645S) according to manufacturer's instructions. Briefly, samples were end-prepped, adaptor ligated and cleaned with Agencourt Ampure XP magnetic beads. Adaptor ligated samples were then barcoded during PCR (10 cycles) and cleaned up.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

22826044

Reads aligned (%)

97.1

Duplicates removed (%)

20.3

Number of peaks

20332 (qval < 1E-05)

mm9

Number of total reads

22826044

Reads aligned (%)

96.9

Duplicates removed (%)

20.3

Number of peaks

20319 (qval < 1E-05)