Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4 cells

cell type

immortalized erythroid cell overexpressing GATA1-ER fusion

treatment

0 hours estradiol

chip antibody

RBP-1 (santa cruz sc899)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Pol II-DNA complexes were isolated with antibody. use Illumina TruSeq ChIP sample preparation kit

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

41475409

Reads aligned (%)

94.0

Duplicates removed (%)

53.1

Number of peaks

10377 (qval < 1E-05)

mm9

Number of total reads

41475409

Reads aligned (%)

93.8

Duplicates removed (%)

53.2

Number of peaks

10371 (qval < 1E-05)