GSM3453241: MCS MCS rep2 [ATAC-seq]; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
Dermal fibroblast
cell type
Fibroblast
transgene
empty vector
passages
10 to 15
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
After defreezing, viability and cell number were quantified with Countes (Invitrogen), and nuclei were extracted from approximately 25,000 live cells with CSK buffer (10 mM PIPES pH 6.8, 100 mM NaCl, 300 mM sucrose, 3 mM MgCl2, 0.1% Triton X-100) on ice for 5 min. Transposase reaction was then carried out. Illumina adaptors were introduced by 9 to 12 total cycles PCR reaction. Amplified DNA fragments were then purified with QIAGEN MinElute PCR Purification Kit and size selected twice with Agencourt AMPure XP (1:1.5 and 1: 0.5 sample to beads; Beckman Coulter, Fullerton, CA, USA).