ChIP-Atlas: SRX4949892

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Chromatin was extracted from frozen liver tissue from each of the individual livers per group. Crosslinking was achieved by adding formaldehyde to a final concentration of 1% for 5 min at room temperature. ChIP was performed using the following ChIP-validated antibodies: H3K27me3 (Abcam Cat # ab6002, 2 μg antibody per 10 μg of sonicated chromatin). Sequencing data was generated from DNA fragments between 600 - 1100 bp (samples G120_M6 through G120_M11) which were digested to 500 bp and then sequenced, or from longer DNA fragments (greater than 1100 bp) which were digested down to 200 bp and then sequenced. Sequencing libraries for samples G120_M12 and G120_M6 were both prepared from the same ChIP'd sample, and sequencing libraries for samples G120_M13 and G120_M9 were both prepared from the same ChIP'd sample. Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, catalog no. E7645) according to the manufacturer's instructions. NEBNext Multiplex Oligos for Illumina (NEB, Set 1; catalog no. E7335, NEB, Set 2; catalog no. E7500) were used for multiplexing.