Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

K27me3_Male_TCPOBOP_27hr

strain

CD-1 (Charles River)

Sex

male

age

7 weeks

treatment

3 mg/kg TCPOBOP_27 h

tissue

liver

chip antibody

H3K27me3 (Abcam #ab6002)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromatin was extracted from frozen liver tissue from each of the individual livers per group. Crosslinking was achieved by adding formaldehyde to a final concentration of 1% for 5 min at room temperature. ChIP was performed using the following ChIP-validated antibodies: H3K27me3 (Abcam Cat # ab6002, 2 μg antibody per 10 μg of sonicated chromatin). This data set was generated from 200 bp fragments and then sequenced. Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, catalog no. E7645) according to the manufacturer's instructions. NEBNext Multiplex Oligos for Illumina (NEB, Set 1; catalog no. E7335, NEB, Set 2; catalog no. E7500) were used for multiplexing.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

23231295

Reads aligned (%)

97.4

Duplicates removed (%)

3.8

Number of peaks

164 (qval < 1E-05)

mm9

Number of total reads

23231295

Reads aligned (%)

97.3

Duplicates removed (%)

4.3

Number of peaks

172 (qval < 1E-05)