GSM3449816: Karpas620-Ctrl; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
KARPAS-620
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
Myeloma cell line
cell line
Karpas620
treatment
none
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were pelleted at 500g for 10 min and lysed in nuclei lysis buffer (10 mM Tris pH 7.4, 10 mM NaCl, 3 mM MgCl2 ,0.1% IGEPAL) Nuclei were pelleted at 500g for 30 min and tagmented with 2 mLTn5 (Illumina) for 60 min at 37°C prior to protein digestion with 2 mL 10 mg/mL Proteinase K at 40°C for 60 minutes. Libraries were size selected with SPRI beads (AMPure, Kapa Biosystems) prior to 12 rounds of PCR amplification.