GSM3446027: Ad-Null 3 ATAC-Seq; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Cardiovascular
Cell type
HUVEC
Primary Tissue
Umbilical Cord
Tissue Diagnosis
Normal
Attributes by original data submitter
Sample
source_name
HUVEC
cell type
Umbilical vein endothelial cells
passage
6-8
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Nuclei were isolated by resuspending ~5000 cells in 50 ul cold lysis buffer (10 mM Tris-HCl, 10 mM NaCl, 3 mM MgCl2, and 0.1% NP-40). The nuclear pellets were resuspended in 50 uL transposition buffer in Nextera DNA Library Preparation Kit (Illumina Inc. San Diego, CA). ATAC libraries were prepared for sequencing using standard Illumina protocols