Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
Granulocytic-monocytic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
GMP +Dox ATAC Seq
strain background
C57BL/6
genotype/variation
r/r iMLL-AF9+
tissue
Bone marrow
cell type
granulocyte monocyte progenitors
preparation
cultured in vitro for two days, Dox added after day one

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
RNA was extracted with TRIzol reagent. For ATAC-Seq, libraries were prepared as previously described by Buenrostro et al. Nature Methods (2013). RNA-Seq libraries were prepared using TruSeq Stranded mRNA Library Preparation Kit from Illumina.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
53161594
Reads aligned (%)
71.7
Duplicates removed (%)
20.7
Number of peaks
46351 (qval < 1E-05)

mm9

Number of total reads
53161594
Reads aligned (%)
71.6
Duplicates removed (%)
20.8
Number of peaks
46259 (qval < 1E-05)

Base call quality data from DBCLS SRA