Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Granulocytic-monocytic progenitors

NA

NA

Sample

source_name

GMP -Dox ATAC Seq

strain background

C57BL/6

genotype/variation

r/r iMLL-AF9+

tissue

Bone marrow

cell type

granulocyte monocyte progenitors

preparation

cultured in vitro for two days, untreated

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

RNA was extracted with TRIzol reagent. For ATAC-Seq, libraries were prepared as previously described by Buenrostro et al. Nature Methods (2013). RNA-Seq libraries were prepared using TruSeq Stranded mRNA Library Preparation Kit from Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

47631678

Reads aligned (%)

72.7

Duplicates removed (%)

18.9

Number of peaks

38306 (qval < 1E-05)

mm9

Number of total reads

47631678

Reads aligned (%)

72.6

Duplicates removed (%)

19.1

Number of peaks

38205 (qval < 1E-05)