Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H3K27ac
Cell type Class
Embryo
Cell type
Mesoderm

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
mesoderm stage 14-17
cell type
mesoderm
chip-antibody
H3K27ac (ab4729, Abcam)
genotype
twi-INTACT; UAS-Nslmb,GFP-Ubx

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Platform Information


instrument_model
Illumina HiSeq 2000

External Database Query

Logs in read processing pipeline


Number of total reads
21971456
Reads aligned (%)
79.3
Duplicates removed (%)
73.0
Number of peaks
7508 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA