Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27me3

Cell type

Cell type Class
Embryo
Cell type
Mesoderm
NA
NA

Attributes by original data submitter

Sample

source_name
mesoderm stage 14-17
cell type
mesoderm
chip-antibody
H3K27me3 (ab6002, Abcam)
genotype
twi-INTACT

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
tissue specific nuclei were extracted vis the INTACT method (Steiner et al., 2012) Samples were amplified using the ThruPLEX DNA-Seq Kit (Rubicon). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit (Part# 0801-0303).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
25894161
Reads aligned (%)
96.1
Duplicates removed (%)
62.3
Number of peaks
3279 (qval < 1E-05)

dm6

Number of total reads
25894161
Reads aligned (%)
93.8
Duplicates removed (%)
67.5
Number of peaks
3031 (qval < 1E-05)

Base call quality data from DBCLS SRA