Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

B cells

NA

NA

Sample

source_name

H3K27ac ChIP-Seq in activated B Cells

cell type

LPS activated splenic B cells CD43-

antibody

rabbit polyclonal anti-H3K27ac antibody (Cat. No. C15410196, Diagenode Inc.)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked with 1% formaldehyde Illumina single and paired end libraries, RNA-seq and DNA-seq Adapter ligation to fragements followed by pcr amplification using indexed primers for Illumnia HiSeq-2500

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

11956818

Reads aligned (%)

89.8

Duplicates removed (%)

43.3

Number of peaks

6099 (qval < 1E-05)

mm9

Number of total reads

11956818

Reads aligned (%)

89.6

Duplicates removed (%)

43.6

Number of peaks

6091 (qval < 1E-05)