Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Uterus

Cell type

HeLa

Primary Tissue

Cervix

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

HeLa

cell type

immortalized cervical cancer cells

treatment

I-BRD9

timepoint

0min

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ChIP-seq samples were purified from sonicated nuclei using antibody-based immunoprecipitation. ATAC-seq samples were generated using Nextera transposase (Illumina) on purified nuclei. ChIP-seq libraries were prepared using the Kapa Hyper Prep Kit according to the manufacturer's protocol.  ATAC-seq libraries were prepared using Nextera chemistry (Illumina). 

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

49101651

Reads aligned (%)

99.1

Duplicates removed (%)

65.9

Number of peaks

6868 (qval < 1E-05)

hg19

Number of total reads

49101651

Reads aligned (%)

99.0

Duplicates removed (%)

66.1

Number of peaks

6886 (qval < 1E-05)