Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BRD9

Cell type

Cell type Class
Uterus
Cell type
HeLa
Primary Tissue
Cervix
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
HeLa
cell type
immortalized cervical cancer cells
treatment
I-BRD9
chip antibody
Rabbit polyclonal anti-BRD9 (Active Motif 61537, 61538)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq samples were purified from sonicated nuclei using antibody-based immunoprecipitation. ATAC-seq samples were generated using Nextera transposase (Illumina) on purified nuclei. ChIP-seq libraries were prepared using the Kapa Hyper Prep Kit according to the manufacturer's protocol.  ATAC-seq libraries were prepared using Nextera chemistry (Illumina). 

Sequencing Platform

instrument_model
NextSeq 500

hg19

Number of total reads
21343726
Reads aligned (%)
179.7
Duplicates removed (%)
7.6
Number of peaks
1927 (qval < 1E-05)

hg38

Number of total reads
21343726
Reads aligned (%)
180.7
Duplicates removed (%)
7.5
Number of peaks
1945 (qval < 1E-05)

Base call quality data from DBCLS SRA