Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pancreas

Cell type

Pancreas

MeSH Description

A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.

Sample

source_name

pancreas

strain

Mixed C57BL6/Sv129

chip antibody

Tri-Methyl-Histone H3 (Lys27) (C36B11), Cell Signaling Technology, Cat# 9733, RRID:AB_2616029

genotype

Bap1 WT;KrasG12D

input used for data normalization

Input#2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with the indicated antibodies. Libraries were prepared according to NEBNext® Ultra™ II DNA Library Prep Kit and sequenced on the Illumina HiSeq4000 platform.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

32142193

Reads aligned (%)

94.9

Duplicates removed (%)

12.8

Number of peaks

127 (qval < 1E-05)

mm9

Number of total reads

32142193

Reads aligned (%)

94.9

Duplicates removed (%)

12.8

Number of peaks

117 (qval < 1E-05)