Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

G1E-ER4+E2

Tissue

Blood

Lineage

cellLine

Description

Gata1 restored erythroid cells, differentiation induced by estradiol (E2)

Sample

source_name

G1E-ER4 erythroid cells

cell type

immortalized erythroid cell overexpressing GATA1-ER fusion

differentiation

differentiated 24 hours with 100nM estradiol

chip antibody

RBP-1 (santa cruz sc899)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and Pol II-DNA complexes were isolated with antibody. use Illumina TruSeq ChIP sample preparation kit size selection using SPRI beads, confirmed ~340bp using Bioanalyzer

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

36800512

Reads aligned (%)

93.5

Duplicates removed (%)

57.7

Number of peaks

8259 (qval < 1E-05)

mm9

Number of total reads

36800512

Reads aligned (%)

93.3

Duplicates removed (%)

57.8

Number of peaks

8258 (qval < 1E-05)