ChIP-Atlas: SRX4925319

Antigen

Cell type Class: Kidney
Cell type: Mesangial Cells
MeSH Description: Smooth muscle-like cells adhering to the wall of the small blood vessels of the KIDNEY at the glomerulus and along the vascular pole of the glomerulus in the JUXTAGLOMERULAR APPARATUS. They are myofibroblasts with contractile and phagocytic properties. These cells and their MESANGIAL EXTRACELLULAR MATRIX constitute the GLOMERULAR MESANGIUM.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: For ChIP-seq, genomic DNA (gDNA) was extracted from cells using a DNeasy Blood and Tissue kit (Qiagen, Chatsworth, CA, USA) according to the manufacturer's instructions. DNA sonication, Illumina's paired-end library preparation and methylated DNA immunoprecipitation were performed as previously described (Feber et al., 2011). Briefly, five micrograms of gDNA were randomly sheared to a median fragment size of 200 bp using a Covaris S220. Sonicated DNAs were subjected to library preparation according to Illumina Paired End libraries preparation protocols. Then methylated DNA immunoprecipitation was performed using the Methylated-DNA IP kit (Zymo Research; Orange, CA, USA). ChIP libraries were prepared for sequencing using standard Illumina protocols.