Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

bone marrow-derived macrophage

strain

C57BL/6J

cell type

bone marrow-derived macrophage

chip antibody

Anti-H3K4me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei from paraformaldehyde-fixed cells and histone-DNA complexes were isolated with antibody. NEBNext® Ultra II DNA Library Prep Kit for Illumina (E7645) was used with 100ng of total DNA for the construction of sequencing libraries

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

53053467

Reads aligned (%)

96.0

Duplicates removed (%)

8.3

Number of peaks

34781 (qval < 1E-05)

mm9

Number of total reads

53053467

Reads aligned (%)

95.8

Duplicates removed (%)

8.5

Number of peaks

34748 (qval < 1E-05)