Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H2A.V

Cell type

Cell type Class
Larvae
Cell type
Salivary glands
NA
NA

Attributes by original data submitter

Sample

source_name
third instar salivary gland from SuUR
strain
SuUR
chip antibody
gamma-H2Av (Rockland)
developmental stage
third instar
tissue
salivary gland

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Salivary glands were dounced and sonicated using a Biorupto 300 (Diagenode) Libraries were constrcted using the NEB Next ChIP seq kit (Part # E6200S) according to manufactures recommendations with indexing primers for multiplexing

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm6

Number of total reads
35975825
Reads aligned (%)
52.4
Duplicates removed (%)
15.9
Number of peaks
1137 (qval < 1E-05)

dm3

Number of total reads
35975825
Reads aligned (%)
52.9
Duplicates removed (%)
16.0
Number of peaks
1006 (qval < 1E-05)

Base call quality data from DBCLS SRA