Crosslinked-cells were sonicated to get 300bp average fragments size followed by pulldown of histone-DNA complexes with HA antibody. Sequencing libraries were prepared by using 1-5 ng of immunoprecipitated DNA via Illumina TruSeq Chip Library Kit according to the manufacturer’s protocol. DNA fragments between 300 and 650 bp were isolated from gel and PCR amplified. Each library was validated by appropriate primer pairs on intended target regions of sgRNA.