GSM3426686: K562 bio-zfp281 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Blood
Cell type
K-562
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Chronic Myelogenous
Attributes by original data submitter
Sample
source_name
hemin induced K562 cell line
cell line
K562
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The cells were cross-linked with 1% formaldehyde for 10 mins at room temperature and quenched with 125 mM glycine. The cells were washed in PBS and then lysed in buffer containing 1% to 0.1% SDS. The genomic DNA was sonicated to 200 to 1000 bps and the biotin-protein-DNA complexes ware precipitated with Dynabeads™ M-280 Streptavidin (Thermo Cat# 11205D) at 4°C for 2 hours, followed by washing and de-crosslinking and DNA purification for subsequent analysis Fragmented IP'ed DNA samples were processed using the Beckman Coulter SPRIworks system to generate libraries. Resulting libraries were sequenced on the HiSeq2000.