GSM3426162: HGSOC-4 H3K27ac ChIP-seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Gonad
Cell type
Ovarian cancer
NA
NA
Attributes by original data submitter
Sample
source_name
Epithelial ovarian cancer
tissue
High-grade serous ovarian cancer
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was incubated at 4°C overnight with 1 µg H3K27ac antibody (DiAGenode, C15410196, Denville, NJ) that had been coupled with protein A and protein G beads (Life Technologies, Carlsbad, CA) ChIP-seq libraries were built using the ThruPLEX-FD Prep Kit (Rubicon Genomics, Ann Arbor, MI). RNA was extracted using the AllPrep kit (QIAgen, Hilden, Germany).