Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Unclassified
Antigen
Unclassified

Cell type

Cell type Class
Gonad
Cell type
Ovarian cancer
NA
NA

Attributes by original data submitter

Sample

source_name
Epithelial ovarian cancer
tissue
Clear cell ovarian cancer

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was incubated at 4°C overnight with 1 µg H3K27ac antibody (DiAGenode, C15410196, Denville, NJ) that had been coupled with protein A and protein G beads (Life Technologies, Carlsbad, CA) ChIP-seq libraries were built using the ThruPLEX-FD Prep Kit (Rubicon Genomics, Ann Arbor, MI). RNA was extracted using the AllPrep kit (QIAgen, Hilden, Germany).

Sequencing Platform

instrument_model
NextSeq 500

hg38

Number of total reads
38176402
Reads aligned (%)
97.3
Duplicates removed (%)
14.5
Number of peaks
29071 (qval < 1E-05)

hg19

Number of total reads
38176402
Reads aligned (%)
96.9
Duplicates removed (%)
15.4
Number of peaks
28974 (qval < 1E-05)

Base call quality data from DBCLS SRA