GSM1342520: ESC control line (ESC4); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H2A.X
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
embryo
cell line
ESC control line (ESC4)
passage
15-20
reprogramming factors
N/A
antibody
H2A.X (Xiao lab)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP DNA samples were obtained with Native ChIP protocol; briefly, chromatin were fractionated and then subject to Mnase digestion to generate mononucleosomes. Then the mononucleosome fraction was extracted with high salt buffer and H2A.X-containing nucleosomes were isolated and enriched with anti-H2A.X antibodies. Libraries were prepared according to Illumina's standard protocol, with instructions accompanying the DNA Sample Kit