GSM1340616: Embryoid Bodies derived from mES cells Jarid2 -/- rescue with Jarid2 BAC H3K27me3 IP, replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
mESCs, differentiated
NA
NA
Attributes by original data submitter
Sample
source_name
embryoid bodies
antibody
H3K27me3 IP (abcam 6002)
cell type
ESC induced to differentiate by the Hanging Drop Method, LIF removal for 8 days
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Formaldehyde cross-linking 1,1% for 10 minutes. Libraries were prepared accordingly to manufactures (TruSeq ChIP sample Prep Kit, Illumina). Sequencing was performed on Hi-Seq 2500 Illumina single reads 100bp.