SRX4796165
GSM3416309: ROSA26-Input; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Hematopoietic progenitors
NA
NA

Attributes by original data submitter

Sample

source_name
MLL-AF9 I-BET resistant cells
strain/background
C57BL/6
cell type
haematopoietic progenitors
condition
I-BET Resistant
treatment
ROSA26_sgRNA
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysate was isolated from formaldehyde cross-linked cells and sonicated to release the chromatin, then immunoprecipitated with antibody. ThruPLEXTM-FD Prep Kit protocol (Rubicon Genomics).

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
14411825
Reads aligned (%)
94.5
Duplicates removed (%)
14.0
Number of peaks
233 (qval < 1E-05)

mm9

Number of total reads
14411825
Reads aligned (%)
94.2
Duplicates removed (%)
14.1
Number of peaks
190 (qval < 1E-05)

Base call quality data from DBCLS SRA