Antigen

Antigen Class

TFs and others

Antigen

Stat1

Cell type

Cell type Class

Cardiovascular

Cell type

Vascular smooth muscle cells

NA

NA

Sample

source_name

aorta

strain/background

C57BL/6

genotype/variation

Wild type

cell type

Vascular Smooth Muscle Cells

stimulation

8h, 1000U/ml, murine IFN alpha; 4h, 1ug/ml, LPS

chip antibody

STAT1 (Cell Signaling Technology, cat# 14994, lot #4)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was carried out as previously described (Siersbaek et al. 2012), with minor modifications. Indexed ChIP DNA libraries were prepared following the TruSeq™ ChIP Library Preparation kit (Illumina, Inc.) protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

9085104

Reads aligned (%)

95.2

Duplicates removed (%)

19.3

Number of peaks

7117 (qval < 1E-05)

mm9

Number of total reads

9085104

Reads aligned (%)

95.1

Duplicates removed (%)

19.4

Number of peaks

7105 (qval < 1E-05)