Antigen

Antigen Class

TFs and others

Antigen

Stat1

Cell type

Cell type Class

Cardiovascular

Cell type

Vascular smooth muscle cells

NA

NA

Sample

source_name

aorta

strain/background

C57BL/6

genotype/variation

Wild type

cell type

Vascular Smooth Muscle Cells

stimulation

none

chip antibody

STAT1 (Cell Signaling Technology, cat# 14994, lot #4)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP was carried out as previously described (Siersbaek et al. 2012), with minor modifications. Indexed ChIP DNA libraries were prepared following the TruSeq™ ChIP Library Preparation kit (Illumina, Inc.) protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

6173280

Reads aligned (%)

86.9

Duplicates removed (%)

26.6

Number of peaks

203 (qval < 1E-05)

mm9

Number of total reads

6173280

Reads aligned (%)

86.8

Duplicates removed (%)

26.7

Number of peaks

201 (qval < 1E-05)