SRX4796010
GSM3416157: IFN alpha+LPS-stimulated Vascular Smooth Muscle Cells (8h/4h) [mm VSMC IFNA8h LPS4h p65 B]; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Rela

Cell type

Cell type Class
Cardiovascular
Cell type
Vascular smooth muscle cells
NA
NA

Attributes by original data submitter

Sample

source_name
aorta
strain/background
C57BL/6
genotype/variation
Wild type
cell type
Vascular Smooth Muscle Cells
stimulation
8h, 1000U/ml, murine IFN alpha; 4h, 1ug/ml, LPS
chip antibody
NFKB p65 (Cell Signaling Technology, cat# 6956, lot# 7)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was carried out as previously described (Siersbaek et al. 2012), with minor modifications. Indexed ChIP DNA libraries were prepared following the TruSeq™ ChIP Library Preparation kit (Illumina, Inc.) protocol.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
28065370
Reads aligned (%)
95.6
Duplicates removed (%)
27.3
Number of peaks
46338 (qval < 1E-05)

mm9

Number of total reads
28065370
Reads aligned (%)
95.6
Duplicates removed (%)
27.4
Number of peaks
46300 (qval < 1E-05)

Base call quality data from DBCLS SRA