GSM3415052: ChIP-seq of 60M-GS cells using anti-H3K27me3 antibody replicate 1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27me3
Cell type
Cell type Class
Gonad
Cell type
Germline stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
Germline stem cells
strain
Green mice (C57BL/6 Tg14(act-EGFP)OsbY01 mice) of DBA/2 background
tissue
testis
genotype/variation
wild type
antibody
anti-H3K27me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde for 10 min. Soluble cell lysates were prepared by sonication or both MNase treatment and sonication. Lysates were incubated with protein A or protein G Dynabeads conjugated with anti-H3K4me3 or anti-H3K27me3 antibodies, and bound fractions were eluted following manufacturer's recommendation. After reversal of cross-linking for elutes and input samples, DNA from input and immunopreciptation fractions was sheared to an average size of approximately 150 bp by ultrasonication, and used for constructing ChIP-seq library.