Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Bone

Cell type

Saos-2

Primary Tissue

Bone

Tissue Diagnosis

Osteosarcoma

Sample

source_name

Human osteosarcoma

cell type

osteoblast cell line

cell line

SaOS-2

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Standard protocol was followed with the following modifications: Reduced the transposase reaction volume from 50 µl to 25 µl, increased the transposase concentration from 1x to 40x, and used 12 cycles of PCR to enrich each library. Libraries were quantified by Q-PCR, Picogreen and LabChip, then were sequenced on the Illumina HiSeq 2500 to 125 bp in pair-ended mode, using the Nextera sequencing primers.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

69692839

Reads aligned (%)

83.2

Duplicates removed (%)

52.3

Number of peaks

39025 (qval < 1E-05)

hg19

Number of total reads

69692839

Reads aligned (%)

83.0

Duplicates removed (%)

52.5

Number of peaks

38655 (qval < 1E-05)