EF-1alpha (promoter)-KDM1A (transgene); 3rd generation after establishment of transgenic line 1
tissue
sperm
chip antibody
anti-H3K4me2 (Millipore 07-030)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Mouse spermatozoa were collected by swim-up procedure. Mono-nucleosomal chromatin samples were prepared by MNase treatment. ChIP experiments with an antibody against H3K4me2 were performed on native chromatin. ChIP-seq and MNase-seq libraries were produced using the Illumina ChIP-seq DNA Sample Prep Kit (Cat# IP-102-1001).