Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Adipocyte
Cell type
Brown adipocytes
NA
NA

Attributes by original data submitter

Sample

source_name
mature brown adipocytes, input
strain/background
C57BL/6J
cell type
immortalized mature brown adipocytes
passages
10
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Antibody against H3K27me3 (Millipore, Cat# 07-449, Lot# 2194165). 7.5 milllion cells were used for each ChIP. Illumina's ChIP-seq Sample Prep kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
27799872
Reads aligned (%)
87.0
Duplicates removed (%)
13.7
Number of peaks
362 (qval < 1E-05)

mm9

Number of total reads
27799872
Reads aligned (%)
86.7
Duplicates removed (%)
13.6
Number of peaks
363 (qval < 1E-05)

Base call quality data from DBCLS SRA