SRX4777951
GSM3407048: P5424 stimulated H3K27Ac replicate1; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
P5424
NA
NA

Attributes by original data submitter

Sample

source_name
P5424_stimulated_ChIP-Seq_H3K27Ac
cell line
P5424
cell type
pro-T-cell line
genotype/variation
Rag2 and P53 knockout
treatment
after PMA-Ionomycin stimulation
chip antibody
H3K27Ac

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
The extraction of total RNA was performed using the RNeasy Plus Mini kit (Qiagen) according to the protocol recommended by the supplier. libraries preparation using the TruSeq RNA Library Prep Kit v2 (Illumina).

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
19463383
Reads aligned (%)
94.6
Duplicates removed (%)
2.7
Number of peaks
7350 (qval < 1E-05)

mm9

Number of total reads
19463383
Reads aligned (%)
94.5
Duplicates removed (%)
2.7
Number of peaks
7317 (qval < 1E-05)

Base call quality data from DBCLS SRA