GSM3405893: cordBlood TCM rep1 [1B-KV]; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
Cord blood, naïve CD8, Anti-CD3 activated, CM
tissue
cord blood
cell type
Central Memory CD8 T cell (TCM)
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
T cells sorted on Aria Fusion (Becton Dickinson, New Jersey, USA) were pelleted and resuspended in a 50-μl reaction cocktail containing 1 μl of 0.5% Digitonin (Promega, Madison, WI, USA), 2.5 μl of Tn5 transposase and 25 μl of TD buffer (Nextera DNA library preparation kit, Illumina, San Diego, CA, USA) and incubated for 30 min at 37°C with gentle shaking. The tagmented DNA was then purified using Minielute PCR Purification kit (Qiagen) and eluted into 11 μl of elution buffer. Transposed DNA was then amplified using 2.5 μl of indexing primers and 25 μl of Nextera PCR master mix according to the PCR protocol previously described (Buenrostro, 2013, Nat. Methods). PCR clean-up was then performed using AMPure XP beads (Beckman Coulter, Brea, CA, USA), and resuspended in 30 μl of resuspension buffer. The libraries were quantified using Qubit fluorometer (Life Technologies), and the size was detemined using TapeStation (Agilent).