Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
HIF1A

Cell type

Cell type Class
Cardiovascular
Cell type
EA.hy926
Tissue
Somatic Cell Hybrid
Cell Type
Endothelial

Attributes by original data submitter

Sample

source_name
EA.Hy926
cell line
EA.Hy926
cell type
Human umbilical vein endothelial cell line
chip antibody
HIF-1alpha (Novus, NB100-134)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. The purified DNA fragments were blunt-ended, ligated to the Solexa paired-end adaptors by using NEBNext Ultra II DNA Library Prep kit and NEBNext Multiplex Oligos kit (NEB, USA) to build the libraries and tested by Agilent 2200.

Sequencing Platform

instrument_model
HiSeq X Ten

hg19

Number of total reads
52990388
Reads aligned (%)
170.9
Duplicates removed (%)
10.8
Number of peaks
2947 (qval < 1E-05)

hg38

Number of total reads
52990388
Reads aligned (%)
173.9
Duplicates removed (%)
10.4
Number of peaks
5198 (qval < 1E-05)

Base call quality data from DBCLS SRA