GSM3402269: 517 Irma Sottsas; Homo sapiens; Bisulfite-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Bisulfite-Seq
Antigen
Bisulfite-Seq
Cell type
Cell type Class
Prostate
Cell type
Prostate cancer
NA
NA
Attributes by original data submitter
Sample
source_name
Human tumor
tissue type
Cancer (Prostate)
Sequenced DNA Library
library_strategy
Bisulfite-Seq
library_source
GENOMIC
library_selection
RANDOM
library_construction_protocol
DNA from cultured human cancer cell lines were extracted using QIAGEN Dneasy Blood & Tissue Kit. Tumor and normal DNA samples from human were extracted from other collaborating centers For NGS using the HiSeq platform, the PCR products were first purified using the QIAquick Gel Purification Kit (Qiagen), followed by sequencing library preparation using Nextera XT DNA Library Preparation Kit (Illumina) according to standard single-end sequencing protocol provided and sequenced following the manufacturer's instructions for the HiSeq 2500 Rapid Run (Illumina) mode to obtain 126 nucleotide read lengths. For NGS using the MiSeq platform, the resulting PCR products were prepared in accordance to the 16S Metagenomic Sequencing Library Preparation guide (15044223 B, Illumina) and were sequenced following the manufacturer's instructions for the MiSeq Reagent Kit v3 (Illumina) to obtain single-end 125 nucleotide read lengths. Bisulfite-Seq (Targeted)