grown in DMEM supplemented with 10% FBS, penicillin and streptomycin, and 2mM L-glutamine
genotype/variation
Pten Null
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP-seq: Lysates were clarified from sonicated nuclei and prtein-DNA complexes were isolated with antibody RNA-seq: RNA was extracted from cell pellets using Qiagen RNeasy Kit. ChIP-seq: 2-10 ng of DNA were subject to end-repair, A-tailing, and ligation with Illumina Truseq adapters using NEB enzymes (NEB), followed by size-selection of 300-400 bp and amplification for 10-15 cycles using the KAPA HiFi Library Amplification Kit (Kapa Biosystems). RNA-seq: Illumina TruSeq RNALibrary Prep Kit v2 (RS 122-2001) was used with 1 ug of total RNA for the construction of sequencing libraries